Abstract :
Three hundreds of Ross 308 chicks at one day-old were used. The experiment was continuousuntil sixth week of age. The objective of this study was to identify genotypes Myostatin gene andits relation with some qualitative characteristics of carcass and different body measurements forbroiler chicken. Three types of restriction enzymes Aci I (Arthrobacter citreus), Bbv I (Bacillusbrevis) and Bbs I (Bacillus brevis), were used in the Restriction fragment length polymorphism(RFLP) analysis.The results of this study can be summarized as follows:The percentages of genotypic frequency for the Myostatin gene by using restriction enzyme Aci I(Arthrobacter citreus) was 32 %, 54% and 14 % for the (GG, GA and AA), respectively. Thedifferences between genotypes was highly significant, furthermore, the effect of the genotypes of theMyostatin gene on carcass yield percentage was significant (P 0.05) too .Effect of the genotypes ofthe Myostatin gene on the body measurement was significant (P 0.05) on the breast width duringthird week and the body length during sixth week. The percentage of genotype distribution for theMyostatin gene by restriction enzyme Bbs I was 6.78%, 86% and 31.36 % for the genotypes. (CC,CT and TT), respectively and the differences between these percentages was highly significant andthe effect of the genotypes of the Myostatin gene on the body measurement was significant (P 0.05)on the leg length during third week and leg length, the body length and comb height during sixthweek. The percentage of genotype distribution for the Myostatin gene by restriction enzyme Bbv Iin samples of studied was 98% and 2 % for the genotypes, (AA and GA) respectively and thedifferences between these percentages was highly significant and the effect of the genotypes of theMyostatin gene on the body measurement was significant (P 0.01) in the leg length and thighthickness during sixth week.
Keywords :
carcass trait , myostatin , body measurements , restriction enzyme , genotypes
