Identification of Agarwood (Aquilaria malaccensis) Chips Incense Smoke and Headspace Volatile Compounds by GC-MS.EI.Q.TOF, SPME

The new hybrid technique which combines gas chromatography and quadruple time of flight mass spectrometry (GC.MS.EI/Q.TOF) were used for the first time in screening and identification of multiple compounds of agarwood chipwood incense smoke and volatile headspace. To explore chemical constituent of Aquilaria malaccensiswhich result in the generation of a complex array of secondary metabolites, we describedgas chromatography method, coupled to accurate mass time-of-flight mass spectrometry (Q.TOFMS.EI) forthe untargeted and comprehensive chemical profiling of chipwood from naturally infected A. quilaria malaccensistrees. Chemical components of agar-wood were extracted by applying a new technique; trapping smoke from burning incense and volatile headspace.The SPME.HS technique, solid phase microextraction (SPME) was employed and gas chromatography (GC.MS.EI/Q.TOF) were used as identification tools for the analysis. Divinylbenzene-carboxen-polydimethyl siloxane (DVB-CAR-PDMS) 50/30 μm fibre was used to extract, and analyze volatile compounds. In total, more than 550 peaks were detected, of which tentative identification of 28 of these compounds wasreported, representing the major compounds that are mentioned in the literature of the oil studies. The total ion chromatogram detected compounds are distributed among a wide range over the chemical families. Furthermore, the feasibility of this methodology wasachieved in grading agarwood chipwood by correlating the obtained chemical profiles of incense smoke and volatile headspace with the extracted oil profile from agarwood, is mentioned in detail; by the way, the major compounds found wasrepresented by β-agarofuran, dihydro 1.27%, γ-cadinene 3.06%, α-bulnesene 3.68%, cadina-1(10),6,8-triene 3.96%, alloaromadendrene 2.09%, cadalene 3.93%, longifolene 2.43% and δ-cadinene 5.01%. This work was one of the very few works that provided information about agarwoodchipwood of A. malaccensiswhich represented a valuable data. Further-more, the study revealed that the characterization of compounds from agarwood chip-wood was highly possible through incense smoke and volatile compounds.