Author/Authors :
ALI, Nouruddin W. Beni-Suef University - Faculty of Pharmacy - Pharmaceutical Analytical Chemistry Department, Egypt , GAMAL, Mohammed Beni-Suef University - Faculty of Pharmacy - Pharmaceutical Analytical Chemistry Department, Egypt , ABDELKAWY, Mohammed Cairo University - Faculty of Pharmacy - Pharmaceutical Analytical Chemistry Department, Egypt , ABOUL-ENEIN, Hassan Y. National Research Centre - Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Egypt
Abstract :
Accurate and sensitive spectrofluorimetric method was developed for determination of diiodohydroxyquinoline in presence of metronidazole. In this method the native fluorescence of diiodohydroxyquinoline in water solvent at λ = 495 nm when excitation was at 250 nm is used for its determination. Linear correlation was obtained in the concentration range of 400 to 900 ng mL^-1. The proposed method was successfully applied for determination of diiodohydroxyquinoline in bulk powder with mean accuracy of 100.21±1.13 or in spiked human plasma with mean accuracy of 100.53±1.42 without interference of metronidazole
