Author/Authors :
Alias, M . F . University of Baghdad - College of Science for Women - Department of Chemistry, Iraq , Hashim, A . J . University of Baghdad - College of Science - Department of Biotechnology, Iraq , Kareem, T . A . University of Baghdad - College of Science for Women - Department of Chemistry, Iraq
Abstract :
The new ligand(OBTMRZ),5/.v[4-phenyl-3(2-propenyi)thio-1,2,4- triazole -5yl] methane was prepared and characterized by vibrational spectra and physical methods,selected metals,which were Cu11, Zn, Mn[l,Co,Fe n and Hg were reacted with ligand to produce new complexes.Solid compounds were isolated and identified through metal analysis,vibrational and electronic spectra,molar conductivity magnetic susceptibility measurements and other techniques depending on the data obtained,chemical formula geometry of complexes were suggested. Other ligands(OXH,TRZH,OTRZ,BTMRZH2)were prepared and select metals(Cun,Zn11andMn)reacted with them to produce complexes. These complexes were characterized by infrared spectroscopy and physical methods . Laccase was produce from fungal isolate P .ostreatus using submerge culture at 30C° pH =6.5 after 96 hour of incubation .The analysis of fungal filtrate using (HPLC) technique shown the presence one peak which indicated , that was partial purified , the flam atomic absorption technique indicated that the enzyme was contains (2Zn lCu,lFe atom) .The optimum conditions for enzyme activity were pH=6 at 60C° and for stability pH=5-6 at 35-60C°,the operational activity of enzyme were pH=6 at 60 C° for 30 min . The effect of salts,indicator (ligands) and their complexes in enzyme activity were studied as following : - Zn complexes were act as enzyme inducer in case of OXH,TRZH, OTRZligands in comparison with Cu complexes but the complexes (BTMRZH2) were reveled opposite behavior with previously ligands. - Enzyme activity was increase with Co ,Fe and Zn complexes by using new ligand OBTMRZ while the activity was inhibited with Hg,Cu and Mn complexes. - Low activation was appeared with organic indicators (ligands ) . - High activation in enzyme activity has been shown with present of Fe,Zn and Co salts,and the highest inhibition was occurred in presence of NaNj and HgCh . The anion part of salts played animported role in enzyme activity ,the activity was increase in presence of NO3*1 group in comparison with chloride group for the same metal.
