Impact of gender and age on the association of the BUD13-ZNF259 rs964184 polymorphism with coronary heart disease

Objective: Coronary heart disease (CHD) is the most common cause of death worldwide. This study aimed to validate the association of the rs964184 polymorphism with the CHD risk and included 874 CHD patients and 776 controls. Methods: rs964184 polymorphism genotyping was performed using Tm-shift polymerase chain reaction. Results: A strong association of the rs964184 polymorphism with CHD was found (genotype: X2=14.365, p=0.001; allele: X2=14.191, p=1.6710–4; power=0.965). Gender analysis revealed a significant association only in males (genotype: X2=12.387, p=0.002; allele: X2=12.404, p=4.3210–4; OR=1.467, 95% CI=1.185–1.817, power=0.945). Age and gender analyses revealed significant associations of the rs964184 polymorphism with CHD in males between the ages of 55 and 65 years (genotype: X2=10.070, p=0.007; allele: X2=10.077, p=0.002; OR=1.706, 95% CI=1.224–2.377, power=0.996) and in females older than 65 years (genotype: X2=9.462, p=0.009; allele: X2=9.560, p=0.002; OR=2.112, 95% CI=1.308–3.412, power=0.994). Further subgroup analysis suggested that rs964184 genotypes were significantly associated with TG levels in the patients (r=0.191, adjusted p=1.05 10–5) and controls (r=0.101, adjusted p=0.026). Conclusion: Our results indicate that both gender and age have great impacts on the association of the rs964184 polymorphism with CHD among Chinese.