Title of article :
ESTIMATION OF GENETIC VARIATIONS IN DIFFERENT TAXA IN BRASSICACEAE BY RAPD an‎d ISSR ANALYSIS
Author/Authors :
Al-Tameme, Huda Jasim M Department of Biology - College of Science for Women - University of Babylon - Babylon, Iraq
Pages :
13
From page :
1
To page :
13
Abstract :
Twelve species from Brassicaceae family were studied using two different molecular techniques:RAPD and ISSR;both of these techniques were used to detect some molecular markers associated with the genotype identification. RAPD results, from using five random primers, revealed 241 amplified fragments, 62 of them were polymorphic (26%). ISSR results showed that out of seven primers, three (ISSR3, UBC807, UBC811) could not amplify the genomic DNA;other primers revealed 183 amplified fragments, 36 of them were polymorphic (20%). The similarity evidenceand dendrogram for the genetic distances of the incorporation between the two techniques showed that the highest similarity was 0.897 between the varieties redcabbage and redornamental cabbage, meanwhile the lowest similarity index was between the varieties redradish and green ornamental cabbage (0.169);thus these RAPD and ISSR markers have the possibility for the identification of species or varieties and the description of genetic variation within the varieties. Furthermore, it could be concluded that the Brassicaceae taxa havea suitable amount of genetic variance and a wide rangein the geneticprinciple of the studied genotypes which can be used for output improvement.
Keywords :
Brassicaceae , Genetic similarity , ISSR , Polymorphism , RAPD
Journal title :
Bulletin of the Iraq Natural History Museum
Serial Year :
2018
Full Text URL :
Record number :
2603008
Link To Document :
بازگشت