Author/Authors :
Yu, Wei Hand-Surgery Department - China-Japan union Hospital of Jilin University, China , Zhao, Guo-qing Anesthesia Department - China-Japan union Hospital of Jilin University, China , Cao, Rang-juan Hand-Surgery Department - China-Japan union Hospital of Jilin University, China , Zhu, Zhi-hua Anesthesia Department - China-Japan union Hospital of Jilin University, China , Li, Kai Anesthesia Department - China-Japan union Hospital of Jilin University, China
Abstract :
Background. Long noncoding RNAs were involved in the processes of diabetes. Our study was aimed to explore clinical potential of
LncRNA NONRATT021972 in diabetic neuropathic pain and investigate detailed mechanisms. Methods. 154 patients with type 2
diabetes were enrolled as experimental group paired with control. Patients without diabetes but neuropathy were enrolled to
explore exclusive role of LncRNA NONRATT021972 in neuropathy. Real-time PCR and ELISA were performed to examine
expression of LncRNA and TNF-α in flood. Neuropathic pain scores were calculated with data from NPQ. Streptozotocin was
used for SD adult male rats to establish diabetes for NONRATT021972 siRNA or saline treatment. Neuropathic pain behaviors
and expression of TNF-α were assessed. Result. Patients with type 2 diabetes had a significantly higher concentration of
LncRNA NONRATT021972 in blood and more severe symptoms of neuropathic pain. LncRNA NONRATT021972 was
positively associated with neuropathic pain scores of type 2 diabetes. TNF-α level increased in patients with type 2
diabetes. Animal experiment showed that LncRNA NONRATT021972 siRNA attenuated inflammation via decreasing TNFα and alleviated neuropathic pain. Conclusion. LncRNA NONRATT021972 increased in type 2 diabetes and was positively
associated with neuropathic pain scoring in type 2 diabetes. LncRNA NONRATT021972 exacerbated neuropathic pain via
TNF-α related pathways
