Author/Authors :
Zhao, Hong Liver Diseases Center - Beijing Ditan Hospital - Capital Medical University, Beijing, China , Wang, Yujie Department of Infectious Diseases - Peking University International Hospital, Beijing, China , Liu, Yufeng Liver Diseases Center - Beijing Ditan Hospital - Capital Medical University, Beijing, China , Hao, Xiaohua Institute of Infectious Diseases - Beijing Ditan Hospital - Capital Medical University, Beijing, China , Wei, Hongshan Institute of Infectious Diseases - Beijing Ditan Hospital - Capital Medical University, Beijing, China , Xie, Wen Liver Diseases Center - Beijing Ditan Hospital - Capital Medical University, Beijing, China
Abstract :
Background. In our previous study, we found that the FAM172A recombinant protein could promote proliferation of L02 cells.However, the underlying mechanisms are still unknown. The present study was aimed at investigating the effect of FAM172Aon proliferation of HepG2 cells and exploring the possible molecular mechanisms and its role in hepatocellular carcinoma(HCC).Methods. Cell proliferation was measured by MTT assay. Western blot test was carried out to investigate themechanism. Rabbit antibodies against FAM172A and membrane proteins isolated from lysate of HepG2 cell were coprecipitatedand the resultant precipitates were analyzed by mass spectrum.Results. The MTT assay showed that recombinant proteinFAM172A isoform 1 (FAM172A-1) could induce HepG2 cell proliferation at the concentration of 10-100 ng/mL, while proteinFAM172A isoform 3 (FAM172A-3) was at the concentration of 80-100 ng/mL. Western blot demonstrated that bothFAM172A-1 and FAM172A-3 could activate the mitogen-activated protein kinase/extracellular signal-regulated protein kinase(MAPK/ERK) pathway and the phosphatidylinositol 3-kinase/threonine-protein kinase (PI3K/Akt) pathway. Mass spectrumanalysis suggested that there were some membrane proteins interacting with FAM172A. Several candidate interacting proteinsmight mediate proliferation signals induced by FAM172A recombinant protein, including seven membrane proteins.Conclusion. In conclusion, FAM172A recombinant protein could induce proliferation of HepG2 cells, in which the MAPK/ERKand PI3K/Akt signaling pathways might be involved. The role of FAM172A in HepG2 cell proliferation also indicated itspossible involvement in HCC. The receptor of FAM172A on cells still needs to be exploited.
