Author/Authors :
Wang, Gaiqing Department of Neurology - Shanxi Medical University, Shanxi, China , Li, Tong Department of Neurology - Shanxi Medical University, Shanxi, China , Duan, Shu-na Department of Neurology - Shanxi Medical University, Shanxi, China , Dong, Liang Department of Neurology - Shanxi Medical University, Shanxi, China , Sun, Xin-gang Department of Neurology - The Second Hospital - Shanxi Medical University, Shanxi, China , Xue, Fang Department of Neurology - The Second Hospital - Shanxi Medical University, Shanxi, China
Abstract :
Background and Purpose. PPAR-γ is a transcriptional factor which is associated with promoting hematoma clearance and reducing
neurological dysfunction after intracerebral hemorrhage (ICH). Haptoglobin- (Hp-) hemoglobin- (Hb-) CD163 acts as a main
pathway to Hb scavenging after ICH. The effect of PPAR-γ on the Hp-Hb-CD163 signaling pathway has not been reported. We
hypothesized that PPAR-γ might protect against ICH-induced neuronal injury via activating the Hp-Hb-CD163 pathway in a
rat ICH model. Methods. 107 Sprague-Dawley rats were used in this research. They were randomly allocated to 4 groups as
follows: sham group, vehicle group, monascin-treated group, and Glivec-treated group. Animals were euthanized at 3 days after
the model was established successfully. We observed the effects of PPAR-γ on the brain water content, hemoglobin levels, and
the expressions of CD163 and Hp in Western blot and real-ime PCR; meanwhile, we measured hematoma volumes and edema
areas by MRI scanning. Result. The results showed that PPAR-γ agonist significantly reduced hematoma volume, brain edema,
and hemoglobin after ICH. It also enhanced CD163 and Hp expression while PPAR-γ antagonist had the opposite effects.
Conclusions. PPAR-γ promotes hematoma clearance and plays a protective role through the Hp-Hb-CD163 pathway in a rat
collagenase infusion ICH model.
Keywords :
PPAR-γ Promotes Hematoma , Clearance through , Haptoglobin-Hemoglobin-CD163 , Rat Model , Intracerebral Hemorrhage
