Author/Authors :
Dezfuli, Neda K. Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Adcock, Ian M. Airways Disease Section - National and Lung Institute - Imperial College London, London, UK , Alipoor, Shamila D. Molecular Medicine Department - Institute of Medical Biotechnology - National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Seyfi, Sharareh Chronic Respiratory Diseases Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Salimi, Babak Chronic Respiratory Diseases Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mafi Golchin, Maryam Department of Biotechnology - Ferdowsi University, Mashhad, Iran , Dalil Roofchayee, Neda Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Varhram, Mohammad Mycobacteriology Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mortaz, Esmaeil Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Background. Lung cancer is one of the leading causes of death worldwide. MicroRNAs (miRNAs) are small noncoding RNAs that
regulate gene expression and may act as both tumor suppressors and as oncogenes. The presence of single nucleotide polymorphisms (SNPs) inside the miRNA genomic region could affect target miRNA maturation, expression, and binding to its target
mRNA and contribute to cancer development. Previous studies on the SNPs Rs2910164 in miR-146a and Rs767649 in miR-155
showed association with non-small cell lung cancer (NSCLC) development. *us, the aim of this study was to detect any
correlation between those SNPs in Iranian NSCLC patients. Methods. In a small cohort study, 165 NSCLC patients and 147
noncancer controls were enrolled between Apr 2015 and Sep 2019 at the Masih Daneshvari Hospital, Tehran, Iran. Allele
frequencies from the genomic DNA of blood cells were studied using PCR-RFLP and their association with the risk of lung cancer
was evaluated. Results. *e rs2910164C allele (OR = 1.56, 95% CI = 1.10–2.21, p = 0.012) and CC genotype (OR = 2.93, 95%
CI = 1.07–7.9, p = 0.034, respectively) were associated with a significantly increased risk for lung cancer compared to that for the
GG genotype. When patients were stratified according to smoking exposure, no association with rs2910164 variants was found.
The AT genotype (OR = 0.57, 95% CI = 0.33–0.99, p = 0.048) and the A allele frequency (OR = 0.58, 95% CI = 0.35–0.98,
p = 0.043) in rs767649 were lower in NSCLC patients in comparison with the control group. In addition, the rs767649 AT
genotype frequency in smoking controls was higher than in smoking NSCLC patients (OR = 0.44, 95% CI = 0.21–0.90, p = 0.024).
No association was found between rs2910164 and rs767649 variants and stage or type of NSCLC. Conclusion. Our finding suggests
that miR-146a rs2910164 and miR-155 rs767649 polymorphisms may be considered as genetic risk factors for the susceptibility to
NSCLC in the Iranian population. However, a larger multicenter study across Iran is needed to confirm these findings.
