Title of article :
Effects of Apatinib on the “Stemness” of Non-Small-Cell Lung Cancer Cells In Vivo and Its Related Mechanisms
Author/Authors :
Yang, Bin Department of Oncology - Hubei Cancer Hospital - Tongji Medical College - Huazhong University of Science and Technology, Wuhan, China , Wang, Yan Department of Oncology - Liyuan Hospital - Tongji Medical College - Huazhong University of Science and Technology, Wuhan, China , Chen, Zhuoying Department of Geriatrics - Liyuan Hospital - Tongji Medical College - Huazhong University of Science and Technology, Wuhan, China , Feng, Yi-Ming Department of Radiology - union Hospital - Tongji Medical College - Huazhong University of Science and Technology, Wuhan, China , Shi, Liang-Liang Cancer Center - union Hospital - Tongji Medical College - Huazhong University of Science and Technology, Wuhan, China
Abstract :
Objective. To investigate the effects of Apatinib on the “stemness” of lung cancer cells in vivo and to explore its related
mechanisms. Methods. A xenograft model of lung cancer cells A549 was established in nude mice and randomized into a control
group (n = 4) and an Apatinib group (n = 4). Tumor tissues were harvested after 2 weeks, and mRNA was extracted to detect
changes in stemness-related genes (CD133, EPCAM, CD13, CD90, ALDH1, CD44, CD45, SOX2, NANOG, and OCT4) and Wnt/
β-catenin, Hedgehog, and Hippo signal pathways. Results. Compared with the control group, the volume and weight of nude mice
treated with Apatinib were different and had statistical significance. Apatinib inhibited the expressions of ABCG2, CD24, ICAM-1,
OCT4, and SOX2 and upregulated the expressions of CD44, CD13, and FOXD3. Apatinib treatment also inhibited the Wnt/
β-catenin, Hedgehog, and Hippo signaling pathways. Conclusion. Apatinib suppressed the growth of non-small-cell lung cancer
cells by repressing the stemness of lung cancer through the inhibition of the Hedgehog, Hippo, and Wnt signaling pathways
Keywords :
Apatinib , Non-Small-Cell Lung , Cancer Cells
Journal title :
Canadian Respiratory Journal
