Author/Authors :
Zhang, Yunli Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Li, Yanming Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Li, Hongling Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Liu, Qingxia Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Wang, Wei Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Jian, Zijuan Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China , Liu, Wenen Department of Clinical Laboratory - Xiangya Hospital of Central South University, Changsha, Hunan, China
Abstract :
Background. Tuberculosis (TB) is usually caused by Mycobacterium tuberculosis, which has the highest mortality rate among
infectious diseases. ,is study is designed to identify the key genes affecting the diagnosis and treatment of TB. Methods.
GSE54992, which included 39 peripheral blood mononuclear cell (PBMC) samples, was extracted from the Gene Expression
Omnibus database. After the samples were classified into type and time groups by limma package, the differentially expressed
genes (DEGs) were analyzed using the Analysis of Variance. Using pheatmap package, hierarchical cluster analysis was performed
for the DEGs. ,en, the key modules correlated with TB were selected using the WGCNA package. Finally, functional and
pathway enrichment analyses were carried out using clusterProfiler package. Results. ,e DEGs in subclusters 3, 6, 7, and 8 were
chosen for further analyses. Based on WGCNA analysis, blue and green modules in type group and pink module in time group
were selected as key modules. From the key modules, 9 (including BAX and ARPC1B) hub genes in type group and 6 (including
DHX36) hub genes in time group were screened. ,rough pathway enrichment analysis, the TNF signaling pathway was enriched
for the green module. Conclusion. DHX36, BAX, and ARPC1B might be key genes acting in the mechanisms of TB. Besides, the
TNF signaling pathway might also be critical for the diagnosis and therapy of the disease.
