Title of article :
Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
Author/Authors :
Lee, Hui Jai Department of Emergency Medicine - Seoul Metropolitan Government - Seoul National University Boramae Medical Center - Seoul 07061 - Republic of Korea , Lee, Sang Yun Department of Physics - Korea Advanced Institute of Science and Technology - Daejeon 24051 - Republic of Korea , Park, HyunJoo Department of Physics - Korea Advanced Institute of Science and Technology - Daejeon 24051 - Republic of Korea , Park, Yong Keun Department of Physics - Korea Advanced Institute of Science and Technology - Daejeon 24051 - Republic of Korea , Shin, Jonghwan Department of Emergency Medicine - Seoul National University College of Medicine - 103 Daehak-ro - Jongno-gu - Seoul 03080 - Republic of Korea
Abstract :
Changes in microcirculation are believed to perform an important role after cardiac arrest. In particular, rheological changes in red blood cells (RBCs) have been observed during and after ischemic-reperfusion injury. Employing three-dimensional laser
interferometric microscopy, we investigated three-dimensional shapes and deformability of RBCs during and after asphyxial
cardiac arrest in rats at the individual cell level. Rat cardiac arrest was induced by asphyxia. Five rats were maintained for 7 min of
no-flow time, and then, cardiopulmonary resuscitation (CPR) was started. Blood samples were obtained before cardiac arrest,
during CPR, and 60 min after return of spontaneous circulation (ROSC). Quantitative phase imaging (QPI) techniques based on
laser interferometry were used to measure the three-dimensional refractive index (RI) tomograms of the RBC, from which
structural and biochemical properties were retrieved. Dynamic membrane fluctuations in the cell membrane were also quantitatively and sensitively measured in order to investigate cell deformability. Mean corpuscular hemoglobin, mean cell volume,
mean corpuscular hemoglobin concentration, and red blood cell distribution width remained unchanged during CPR and after
ROSC compared with those before cardiac arrest. QPI results revealed that RBC membrane fluctuations, sphericity, and surface
area did not change significantly during CPR or after ROSC compared with initial values. In conclusion, no three-dimensional shapes and cell deformability changes in RBCs were detected.
Keywords :
Three-Dimensional Shapes , Cell Deformability , Rat , Red Blood Cells , Asphyxial Cardiac Arrest , RBCs , CPR
Journal title :
Emergency Medicine International