Title of article :
Klebsiella pneumoniae Planktonic and Biofilm Reduction by Different Plant Extracts: In Vitro Study
Author/Authors :
Paula-Ramos, Lucas Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Eduardo da Rocha Santos, Carlos Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Camargo Reis Mello, Daphne Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Nishiama Theodoro, Lígia Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , De Oliveira, Felipe Eduardo Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Back Brito, Graziella N Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Campos Junqueira, Juliana Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Olavo Cardoso Jorge, Antonio Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil , Dias de Oliveira, Luciane Department of Biosciences and Oral Diagnosis - Sao Paulo State University (Unesp) - Institute of Science and Technology - ˜ Sao Jos ˜ e dos Campos - Francisco Jos ´ e Longo 777 - S ´ ao Dimas - 12245-000 S ˜ ao Jos ˜ e dos Campos - SP - Brazil
Abstract :
This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at
107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (𝑛 = 10) and after 48 h at 37∘
C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical
significant reduction (𝑝 < 0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions
in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with
P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.
Keywords :
Klebsiella pneumoniae Planktonic , Biofilm Reduction , Plant Extracts , In Vitro Study
Journal title :
The Scientific World Journal
