A New Approach for the AFM-Based Mechanical Characterization of Biological Samples

The AFM nanoindentation technique is a powerful tool for the mechanical characterization of biological samples at the nanoscale.The data analysis of the experimentally obtained results is usually performed using the Hertzian contact mechanics. However, theaforementioned theory can be applied only in cases that the sample is homogeneous and isotropic and presents a linear elasticresponse. However, biological samples often present depth-dependent mechanical properties, and the Hertzian analysis cannotbe used. Thus, in this paper, a different approach is presented, based on a new physical quantity used for the determination ofthe mechanical properties at the nanoscale. The aforementioned physical quantity is the work done by the indenter per unitvolume. The advantages of the presented analysis are significant since the abovementioned magnitude can be used to examine ifa sample can be approximated to an elastic half-space. If this approximation is valid, then the new proposed method enables theaccurate calculation of Young’s modulus. Additionally, it can be used to explore the mechanical properties of samples that arecharacterized by a depth-dependent mechanical behavior. In conclusion, the proposed analysis presents an accurate yet simpletechnique for the determination of the mechanical properties of biological samples at the nanoscale that can be also usedbeyond the Hertzian limit