Author/Authors :
Chengxi, Hu Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Lei, Zhang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Huihui, Yang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China , Runguang, Sun Schools of Physics and Information Technology - Shaanxi Normal University - Xi’an - Shaanxi, China , Changchun, Hao Schools of Physics and Information Technology - Shaanxi Normal University - Xi’an - Shaanxi, China , Huihui, Yang Department of Experimental Teaching Center for Optoelectronic Science and Information Engineering - Xi’an Aeronautical University - Xi’an, - Shaanxi, China
Abstract :
Toinvestigatethestabilityanddynamiccharacteristicsofmonolayeradsorbedonunsaturatedlipiddioleoylphosphatidylcholine (DOPC) withvaryingconcentrationsofmyelinbasicprotein(MBP), thesystemisstudiedbyapplyingLangmuirtechniqueandmakingatomicforcemicroscope(AFM)observation,whichisbasedonthemassconservationequationanalysismethodreferredtointhethermodynamicstheory.Asindicatedbysurfacepressure-meanmoleculararea (π−A) and surfacepressure-adsorptiontime(π−T) isotherms,thephysicalpropertiesofmonolayerderivedfromtheinteractionofvaryingconcentrationsofMBPwithliquidcrystallineunsaturatedlipid DOPCmoleculeswerequalitativelystudied.AsrevealedbysurfacemorphologyanalysiswithAFM,themicroregionwasexpandedastheconcentration of MBPinthesubphasewasontheincrease,suggestingthathydrophobicinteractionsledtotheMBPinsertion,thuscausingaccumulationoftheMBPonthesurfaceofthemonolayer. Experimentalresultshavedemonstratedthatthepartitioncoefficientoftheinteractionbetween MBP andunsaturatedphospholipid DOP CandthemolecularareaofMBPadsorbedonthemonolayerfilmwascalculatedusingthemassconservationequation.Inaddition,notonlydoesthevaryingconcentrationofMBPinthesubphaseexertssignificanteffectsonthearrangementandconformationof DOPCmonolayer,italsohascertainguidingsignificancetoexploringthestructuralchangestobiofilmsupramolecularaggregatesaswellasthepathogenesisandtreatmentofrelateddiseases.
Keywords :
Thermodynamic Analysis , Myelin Basic Protein Adsorbed , Liquid Crystalline Dioleoylphosphatidylcholine Monolayer , AFM , DOPC
