Title of article
Tumor Digital Masking Allows Precise Patient Triaging: A StudyBased on Ki-67 Scoring in Gastrointestinal Stromal Tumors
Author/Authors
Lewitowicz, Piotr Department of Pathology - Faculty of Medicine and Health Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Matykiewicz, Jaroslaw Department of Surgery and Surgical Nursing - Faculty of Medicine and Health Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Koziel, Dorota Department of Surgery and Surgical Nursing - Faculty of Medicine and Health Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Gluszek , Stanisław Department of Surgery and Surgical Nursing - Faculty of Medicine and Health Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Wawrzycka, Iwona Department of Surgery and Surgical Nursing - Faculty of Medicine and Health Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Chrapek, Magdalena Department of Probability Theory and Statistics - Institute of Mathematics - The Faculty of Mathematics and Natural Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Horecka-Lewitowicz, Agata Department of Public Health, Faculty of Medicine and Heath Sciences - Jan Kochanowski University in Kielce - Kielce, Poland , Gluszek-Osuch, Martyna Department of Public Health, Faculty of Medicine and Heath Sciences - Jan Kochanowski University in Kielce - Kielce, Poland
Pages
9
From page
1
To page
9
Abstract
Technologicaladvancesconstantlyprovidecutting-edgetoolsthatenhancetheprogressofdiagnosticcapabilities. Gastrointestinalstromaltumorsbelongtoafamilyofmesenchymaltumorswherepatienttriagingisstillbasedontraditionalcriteriasuchasmitoticcount,tumorsize,andtumorlocation.Limitationsofthehumaneyeandrandomnessinchoiceofareaformitoticfigurecountingcompelustoseekmoreobjectivesolutionssuchasdigitalimageanalysis.Presently,thelabellingofproliferativeactivityisbecomingaroutinetaskamidstmanycancers.Thepurposeofthepresentstudywastocomparethetraditionalmethodofpredictionbasedonmitoticratiowithdigitalimageanalysisofcellcycle-dependentproteins.Methods.Fifty-seveneligiblecaseswereenrolled.Furthermore,adigitalanalysisofpreviouslyperformedwholetissuesectionimmunohistochemicalassayswasexecuted.Digitallabellingcoveredbothhotspotsandnot-hotspotsequally.Results.Wenotedasignificantdiversityofproliferativeactivities,andconsequently,theresultspointedto6.5% ofKi-67,countedinhotspots,astheoptimalcut-offforlow–high-gradeGIST.ROCanalysis (AUC=0.913;95%CI:0.828–0.997,p<000001)andoddsratio(OR=40.0,95%CI:6.7–237.3,p<00001)pointedtoKi-6716%asthecut-offforveryhigh-grade(groups5–6)cases. Withhelpofatumordigitalmap,werevealedpossibleerrorsresultingfromawrongchoiceoffieldforanalysis.WeconfirmedthatKi-67scoresareinlinewiththelevelofintracellularmetabolismthatcouldbeusedastheadditionalbiomarker. Conclusions.Tumordigitalmaskingisverypromisingsolutionforrepeatableandobjectivelabelling.Softwareadjustmentsofnuclearshape,outlines,size,etc. arehelpfultoomitotherKi-67-positivecellsespeciallysmalllymphocytes. OurresultspointedtoKi-67asagoodbiomarkerin GIST,butconcurrently,wenotedsignificantdifferencesinuseddigitalapproacheswhichcouldleadtounequivocalresults
Keywords
Tumor Digital Masking , Precise Patient Triaging , Study Based , Ki-67 Scoring , Gastrointestinal Stromal Tumors , GIST
Journal title
Scanning
Serial Year
2018
Record number
2613583
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