Author/Authors :
Ercolin, Anna Carolina Mazeto Veterinary Medicine Department - Faculty of Animal Sciences and Food Engineering - Universidade de Sao Paulo (USP), Pirassununga-SP, Brazil , Roballo, Kelly Cristine Santos Faculty of Animal Sciences and Food Engineering - USP, Pirassununga-SP, Brazil , Casals, Juliana Barbosa Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Pieri, Naira Caroline Godoy Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Souza, Aline Fernanda Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Barreto, Rodrigo da Silva Nunes Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Bressan, Fabiana Fernandes Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Feitosa, Matheus Levi Tajra Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Miglino, Maria Angélica Department of Veterinary Surgery - Faculty of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Meirelles, Flávio Vieira Faculty of Animal Sciences and Food Engineering - USP, Pirassununga-SP, Brazil , Ambrósio, Carlos Eduardo Faculty of Animal Sciences and Food Engineering - USP, Pirassununga-SP, Brazil
Abstract :
PURPOSE: To describe a new technique for isolation of a mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits.
METHODS: Olfactory stem cells (OSCs) were retrieved from under the cribriform plate of the Ethmoid bone. Several assays were accomplished to characterize the cell population and attest its viability in vitro. The cells were submitted to flow cytometry with the antibodies CD34, CD45, CD73, CD79, CD90 and CD105 and also they were induced to differentiate in three lineages. Functional evaluation involved analysis of in vitro growth behavior, colony forming unit like fibroblasts (CFU-f) and cryopreservation response. Further transduction with Green Fluorescent Protein (GFP) was also performed.
RESULTS: The OSCs showed mesenchymal features, as positive response to CD34, CD73 and CD90 antibodies and plasticity. Additionally, these cells have high proliferated rate, and they could be cultured through many passages and kept the ability to proliferate and differentiate after cryopreservation. The positive response to the transduction signalizes the possibility of cellular tracking in vivo. This is a desirable feature in case those cells are used for pre-clinical trials.
CONCLUSION: The cells harvested were mesenchymal stem cells and the technique described is therefore efficient for rabbit olfactory stem cells isolation.
