Author/Authors :
Liu, Songhao MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology - College of Biophotonics - South China Normal University - Guangzhou, China , Qiu, Xuejun MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology - College of Biophotonics - South China Normal University - Guangzhou, China , Huang, Hanchuan MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology - College of Biophotonics - South China Normal University - Guangzhou, China , Zhuang, Zhengfei MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology - College of Biophotonics - South China Normal University - Guangzhou, China , Guo, Zhouyi MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology - College of Biophotonics - South China Normal University - Guangzhou, China , Huang, Zhitong Guzhen Productivity Promotion Center - Zhongshan, China
Abstract :
Red light-emitting diodes (LED) were used to irradiate the isolated hypertension hemoglobin (Hb) and Raman spectra differencewas recorded using confocal micro-Raman spectroscopy. Differences were observed between the controlled and irradiated Hb bycomparing the spectra records. The Raman spectrum at the 1399 cm−1band decreased following prolonged LED irradiation. Theintensity of the 1639 cm−1band decreased dramatically in the first five minutes and then gradually increased in a time-dependentmanner. This observation indicated that LED irradiation increased the ability of oxygen binding in Hb. The appearance of the hemeaggregation band at 1399 cm−1,inadditiontotheoxygenmarkerbandat1639cm−1, indicated that, in our study, 30 min of irradiationwith 15.0 mW was suitable for inhibiting heme aggregation and enhancing the oxygen-carrying capacity of Hb. Principal componentanalysis showed a one-to-one relationship between irradiated Hb at different time points and the corresponding Raman spectra.Our approach could be used to analyze the hemoglobin from patients with confocal micro-Raman spectroscopy and is helpful fordeveloping new nondrug hypertension therapy.
Keywords :
Red Light-Emitting , Diodes Irradiation , Hemoglobin , Potential Hypertension Treatment , Confocal Micro-Raman Spectroscopy
