Author/Authors :
He, Yongxiong Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Cao, Yongfei Guizhou Provincial Orthopedics Hospital - Shangchong South Road - Nanming District - Guiyang City - Guizhou Province, China , Wang, Xiaolei Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Jisiguleng, Wu Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Tao, Mingkai Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Liu, Jianfeng Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Wang, Fei Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Chao, Lemeng Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Wang, Wenjun Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Li, Pengfei Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Fu, Haiping Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Xing, Wei Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Zhu, Zhibo Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Huan, Yanqiang Department of Spine Surgery - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China , Yuan, Hongwei Department of Pathology - Inner Mongolia People’s Hospital - Hohhot - Inner Mongolia, China
Abstract :
Breast cancer (BC) had been one of the deadliest types of cancers in women worldwide. More than 65% of advanced-stage BC
patients were identified to have bone metastasis. However, the molecular mechanisms involved in the BC spinal metastases
remained largely unclear. This study screened dysregulated genes in the progression of BC spinal metastases by analyzing
GSE22358. Moreover, we constructed PPI networks to identify key regulators in this progression. Bioinformatics analysis
showed that these key regulators were involved in regulating the metabolic process, cell proliferation, Toll-like receptor and
RIG-I-like receptor signaling, and mRNA surveillance. Furthermore, our analysis revealed that key regulators, including C1QB,
CEP55, HIST1H2BO, IFI6, KIAA0101, PBK, SPAG5, SPP1, DCN, FZD7, KRT5, and TGFBR3, were correlated to the OS time in
BC patients. In addition, we analyzed TCGA database to further confirm the expression levels of these hub genes in breast
cancer. Our results showed that these regulators were significantly differentially expressed in breast cancer, which were
consistent with GSE22358 dataset analysis. Furthermore, our analysis demonstrated that CEP55 was remarkably upregulated in
the advanced stage of breast cancer compared to the stage I breast cancer sample and was significantly upregulated in triplenegative breast cancers (TNBC) compared to other types of breast cancers, including luminal and HER2-positive cancers,
demonstrating CEP55 may have a regulatory role in TNBC. Finally, our results showed that CEP55 was the most highly
expressed in Basal-like 1 TNBC and Basal-like 2 TNBC samples but the most lowly expressed in mesenchymal stem-like TNBC
samples. Although more studies are still needed to understand the functions of key regulators in BC, this study provides useful
information to understand the mechanisms underlying BC spinal metastases.
