Title of article :
Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line
Author/Authors :
Tri Endharti, Agustina Department of Parasitology - Faculty of Medicine - Brawijaya University - Malang - Indonesia - Biomedical Central Laboratory Faculty of Medicine - Brawijaya University - Malang - Indonesia - Doctoral Program in Biomedical Sciences - Faculty of Medicine - Brawijaya University - Indonesia , Purnamasari, Yeni Magister in Biomedical Science Program - Medical Faculty - Brawijaya University - Malang - Indonesia , Primasari, Renata Magister in Biomedical Science Program - Medical Faculty - Brawijaya University - Malang - Indonesia , Poeranto, Sri Department of Parasitology - Faculty of Medicine - Brawijaya University - Malang - Indonesia , Permana, Sofy Department of Biology - Faculty of Mathematics and Natural Sciences - Brawijaya University - Malang - Indonesia
Pages :
9
From page :
1
To page :
9
Abstract :
Coelomic fluid of Lumbricus rubellus (CFL) has attracted interest due to its pharmacological properties, including antitumor effect. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. This study aims to investigate whether the combination of CFL and 5-fluorouracil could reduce FAK protein level and iCa2+ and enhance p21 level. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. After 24 hours of treatment, it was necessary to assess the percentage of apoptosis, FAK, and p21 protein expression by flow cytometry. iCa2+ concentration was measured using immunofluorescence. The combination therapy of CFL with 5-fluorouracil potently suppressed six treatment groups were included in this study. HT-29 cell lines were cultured and divided into six groups: group 1 was treated with vehicle (negative control), groups 2-5 were treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5-fluorouracil, and group 6 was treated with CFL 20 µg/ml, the progression of colorectal cancer. Combination of CFL and 5-fluorouracil significantly decreased FAK expression (p<0.05), iCa2+ (p<0.05), and increased p21 expression (p<0.05) in HT-29 cells. Our results suggest that CFL has an anticancer potential in colorectal cancer when combined with 5-fluorouracil.
Keywords :
Coelomic fuid of Lumbricus rubellus(CFL) , HT-29 cell lines , Synergistically Enhances Cytotoxic , 5-Fluorouracil through Modulation , Focal Adhesion Kinase
Journal title :
The Scientific World Journal
Serial Year :
2019
Full Text URL :
Record number :
2615244
Link To Document :
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