Author/Authors :
Éboli, Lígia Patrícia de Carvalho Batista Universidade Federal de São Paulo (UNIFESP), Brazil , Netto, Alcides Augusto Salzedas Department of Surgery - UNIFESP, Sao Paulo-SP, Brazil , Azevedo, Ramiro Antero de Pediatric Department - UNIFESP, Sao Paulo-SP, Brazil , Lanzoni, Valéria Pereira Department of Pathology - UNIFESP, Sao Paulo-SP, Brazil , Paula, Tatiana Sugayama de School of Medicine - UNIFESP, Sao Paulo-SP, Brazil , Goldenberg, Alberto Department of Surgery - UNIFESP, Sao Paulo-SP, Brazil , Gonzalez, Adriano Miziara Department of Surgery - UNIFESP, Sao Paulo-SP, Brazil
Abstract :
PURPOSE:
To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation.
METHODS:
Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) .
RESULTS:
Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed.
CONCLUSION:
King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.
Keywords :
Liver Failure , Acute , Galactosamine , Models , Animal , Rats
