Author/Authors :
Topolyan, A.P. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Belyaeva, M.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Bykov, E.E. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Coodan, P.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Rogozhin, E.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Strizhevskaya, D.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Ivanova, O.M. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Ustinov, A.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Mikhura, I.V. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Prokhorenko, I.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Korshun, V.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia , Formanovsky, A.A. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, russia
Abstract :
Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6-dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures.
Keywords :
aminoglycoside antibiotics , mass-spectrometry , trityl cation , HPLC
