Author/Authors :
Baymukhametov, T.N National Research Center «Kurchatov Institute», Moscow, Russia , Chesnokov, Y.M National Research Center «Kurchatov Institute», Moscow, Russia , Pichkur, E.B National Research Center «Kurchatov Institute», Moscow, Russia , Boyko, K.M National Research Center «Kurchatov Institute», Moscow, Russia , Tikhonova, T.V Bach Institute of Biochemistry - Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia , Myasnikov, A.G Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC «Kurchatov Institute», Russia , Vasiliev, A.L National Research Center «Kurchatov Institute», Moscow, Russia , Lipkin, A.V National Research Center «Kurchatov Institute», Moscow, Russia , Popov, V.O National Research Center «Kurchatov Institute», Moscow, Russia , Kovalchuk, M.V National Research Center «Kurchatov Institute», Moscow, Russia
Abstract :
structure of cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducenswas determined by cryo-electron microscopy (cryo-EM) at a 2.56 Å resolution. Possible structural heterogeneity of the enzyme was assessed. The backbone and side-chain orientations in the cryo-EM-based model are, in gen-eral, similar to those in the high-resolution X-ray diffraction structure of this enzyme.
Keywords :
cytochrome cnitrite reductase , structural biology , high-resolution cryo-electron microscopy , single particle analysis
