Title of article :
Gallium-68 Labeled Iron Oxide Nanoparticles Coated with 2,3-Dicarboxypropane-1,1-diphosphonic Acid as a Potential PET/MR Imaging Agent: A Proof-of-Concept Study
Author/Authors :
Karageorgou, Maria-Argyro Department of Solid State Physics - NKUA - Athens, Greece , Vranješ-DjuriT, Sanja University of Belgrade - Mike Petrovica Alasa - Belgrade, Serbia , RadoviT, Magdalena University of Belgrade - Mike Petrovica Alasa - Belgrade, Serbia , Lyberopoulou, Anna Department of Basic Medical Science - Laboratory of Biology - School of Medicine - NKUA - Athens, Greece , AntiT, Bratislav University of Belgrade - Mike Petrovica Alasa - Belgrade, Serbia , Rouchota, Maritina Department of Medical Physics - School of Medicine - University of Patras - Rion, Greece , Gazouli, Maria Department of Basic Medical Science - Laboratory of Biology - School of Medicine - NKUA - Athens, Greece , Loudos, George Department of Medical Instruments Technology - Technological Educational Institute of Athens - Spyridonos Street - Egaleo, Greece , Xanthopoulos, Stavros National Center for Scientific Research “Demokritos” - Aghia Paraskevi - Athens, Greece , Sideratou, Zili National Center for Scientific Research “Demokritos” - Aghia Paraskevi - Athens, Greece , Stamopoulos, Dimosthenis Department of Solid State Physics - NKUA - Athens, Greece , Bouziotis, Penelope National Center for Scientific Research “Demokritos” - Aghia Paraskevi - Athens, Greece , Tsoukalas, Charalampos National Center for Scientific Research “Demokritos” - Aghia Paraskevi - Athens, Greece
Abstract :
The aim of this study was to develop a dual-modality PET/MR imaging probe by radiolabeling iron oxide magnetic nanoparticles
(IONPs), surface functionalized with water soluble stabilizer 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), with the positron
emitter Gallium-68. Magnetite nanoparticles (Fe3O4 MNPs) were synthesized via coprecipitation method and were stabilized
with DPD. The Fe3O4-DPD MNPs were characterized based on their structure, morphology, size, surface charge, and magnetic
properties. In vitro cytotoxicity studies showed reduced toxicity in normal cells, compared to cancer cells. Fe3O4-DPD MNPs were
successfully labeled with Gallium-68 at high radiochemical purity (>91%) and their stability in human serum and in PBS was
demonstrated, along with their further characterization on size and magnetic properties. The ex vivo biodistribution studies in
normal Swiss mice showed high uptake in the liver followed by spleen. The acquired PET images were in accordance with the ex
vivo biodistribution results. Our findings indicate that 68Ga-Fe3O4-DPD MNPs could serve as an important diagnostic tool for
biomedical imaging.
Keywords :
Gallium-68 , Oxide , PET/MR , IONP
