Author/Authors :
Shramova, E.I. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, russia , Proshkina, G.M. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, russia , Chumakov, S.P. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, russia , Khodarovich, Y.M. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, russia , Deyev, S.M. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, russia
Abstract :
In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, fu-rimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.
Keywords :
bioluminescence resonance energy transfer , NanoLuc luciferase , flavoprotein miniSOG , photody-namic therapy
