Author/Authors :
Cunha, Joao Paulo da Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil , Lizarte, Fermino Sanches Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil , Novais, Paulo Cezar Department of Health Sciences - Universidade de Marília, Brazil , Gattas, Daniela Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Ribeirao Preto-SP, Brazil , Carvalho, Camila Albuquerque Mello de Federal University of Alagoas, Alagoas, Brazil , Tirapelli, Daniela Pretti da Cunha Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Ribeirao Preto-SP, Brazil , Molina, Carlos Augusto Fernandes Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Ribeirao Preto-SP, Brazil , Tirapelli, Luis Fernando Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Ribeirao Preto-SP, Brazil , Tucci, Silvio Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Ribeirao Preto-SP, Brazil
Abstract :
Purpose:
To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies.
Methods:
Forty eight Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood.
Results:
Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups.
Conclusion:
The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.
Keywords :
Alcoholism , Diabetes Mellitus , Erectile Dysfunction , Rats
