Author/Authors :
Kornilaeva, G.V Gamaleya Center for Epidemiology and Microbiology. Gamaleya Str.1 8, Moscow, Russia , Siniavin, A.E Gamaleya Center for Epidemiology and Microbiology. Gamaleya Str.1 8, Moscow, Russia , Schultz, A Fraunhofer Institut fuer Biomedizinische Technik (IBMT), Germany , Germann. A Fraunhofer Institut fuer Biomedizinische Technik (IBMT), Germany , Moog, C Université de Strasbourg, Strasbourg, France , von Briesen, H Fraunhofer Institut fuer Biomedizinische Technik (IBMT), Germany , Turgiev, A.S Gamaleya Center for Epidemiology and Microbiology. Gamaleya Str.1 8, Moscow, Russia , Karamov, E.V Gamaleya Center for Epidemiology and Microbiology. Gamaleya Str.1 8, Moscow, Russia
Abstract :
The anti-HIV activity of a new humic substance-derived preparation has been studied in individual pools of immune cells (CD4+ T lymphocytes, macrophages, dendritic cells). Near-complete inhibition of the HIV infection (by more than 90%) was achieved by treating each of the abovementioned cell types with non-toxic concentrations of the preparation. The inhibitory effect demonstrates the possibility of preventing the depletion of a significant portion of functionally important immune cells. A comparative study of infection inhibition in individual cell pools has allowed us to reveal the differences in the preparation’s effectiveness in each of the cell populations. A R5-tropic HIV-1 infection in macrophages exhibited maximum sensitivity to the preparation: 90% and 50% inhibition of the infection were observed in the presence of concentrations as low as 1.4 and 0.35 μg/ml, respectively. A 15- and 19-fold higher concentration was required to achieve the same extent of inhibition in dendritic cells infected with the same strain. The effectiveness of the drug in CD4 + T lymphocytes is quite comparable to its effectiveness in macrophages. The drug is universally effective for both the T- and M-tropic variants of HIV-1.
Keywords :
T- and M-tropic HIV variants , dendritic cells , macrophages , CD4-positive T lymphocytes , suppression of HIV infection , humic derivative
