Title of article
Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses
Author/Authors
rkhipenko, M.V Department of Virology - Lomonosov Moscow State University, Moscow, Russia , Nikitin, N.A Department of Virology - Lomonosov Moscow State University, Moscow, Russia , Baranov, O.A Department of Virology - Lomonosov Moscow State University, Moscow, Russia , Evtushenko, E.A Department of Virology - Lomonosov Moscow State University, Moscow, Russia , Atabekov, J.G Department of Virology - Lomonosov Moscow State University, Moscow, Russia
Pages
6
From page
73
To page
78
Abstract
VOL. 11 No 4 (43) 2019 | ACTA NATURAE | 73RESEARCH ARTICLES
ABSTRACT Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (iso-electric point) has been determined for most plant viruses. Here, we report on the first application of positively charged magnetic nanoparticles for mapping the surface charge distribution of helical plant viruses. The charge was demonstrated to be unevenly distributed on the surface of viruses belonging to different taxonomic groups, with the negative charge being predominantly located at one end of the virions. This charge distribution is mainly controlled by viral RNA.
Keywords
surface charge mapping , magnetic nanoparticles , plant viruses
Journal title
Acta Naturae
Serial Year
2019
Record number
2617112
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