Author/Authors :
Nazarov, P.A Belozersky Institute of Physico-Chemical Biology - Lomonosov Moscow State University, Moscow, Russia , Kotova, E.A Belozersky Institute of Physico-Chemical Biology - Lomonosov Moscow State University, Moscow, Russia , Skulachev, V.P Belozersky Institute of Physico-Chemical Biology - Lomonosov Moscow State University, Moscow, Russia , Antonenko, Y.N Belozersky Institute of Physico-Chemical Biology - Lomonosov Moscow State University, Moscow, Russia
Abstract :
SkQ1, a novel antibiotic targeting bacterial bioenergetics, is highly effective against both gram-pos-itive and gram-negative bacteria. However, some gram-negative bacteria, such as Escherichia coli and Klebsiella pneumoniae, are highly resistant to it. In different gram-negative bacteria, this resistance is associated with the identity of their AcrB transporter protein sequence with the sequence of the AcrB protein from E. coli. SkQ1 is expelled from E. coli cells by the AcrAB-TolC multidrug efflux pump. In this study, we demonstrate that SkQ1 resistance in E. coli, in contrast to chloramphenicol resistance, does not depend on the presence of the multidrug efflux pump accessory protein AcrZ.
Keywords :
multidrug resistance , AcrAB-TolC efflux pump , AcrZ , SkQ1
