Author/Authors :
Kolesnikova, E.A Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Brusnigina, N.F Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Makhova, M.A Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Alekseeva, A.E Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia
Abstract :
The genome structure of three ciprofloxacin-resistant Mycoplasma hominis clinical isolates was
studied using next-generation sequencing on the Illumina platform. The protein sequences of the studied
Mycoplasma strains were found to have a high degree of homology. Mycoplasma hominis (M45, M57, MH1866)
was shown to have limited biosynthetic capabilities, associated with the predominance of the genes encoding
the proteins involved in catabolic processes. Multiple single-nucleotide substitutions causing intraspecific
polymorphism of Mycoplasma hominis were found. The genes encoding the efflux systems – ABC transporters
(the ATP-binding cassette superfamily) and proteins of the MATE (multidrug and toxic compound extrusion)
family – were identified. The molecular mechanism of ciprofloxacin resistance of the Mycoplasma hominis M45
and M57 isolates was found to be associated with the Ser83Leu substitution in DNA gyrase subunit A. In the Mycoplasma
hominis MH1866 isolate it was related to the Lys144Arg substitution in topoisomerase IV subunit A.
Keywords :
MATE , ABC transporters , gyrA and parC genes , antibiotic resistance mechanisms , genome structure , Mycoplasma hominis
