• Title of article

    The Genome Structure of Ciprofloxacin- Resistant Mycoplasma Hominis Clinical Isolates

  • Author/Authors

    Kolesnikova, E.A Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Brusnigina, N.F Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Makhova, M.A Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia , Alekseeva, A.E Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology - Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, Russia

  • Pages
    7
  • From page
    56
  • To page
    62
  • Abstract
    The genome structure of three ciprofloxacin-resistant Mycoplasma hominis clinical isolates was studied using next-generation sequencing on the Illumina platform. The protein sequences of the studied Mycoplasma strains were found to have a high degree of homology. Mycoplasma hominis (M45, M57, MH1866) was shown to have limited biosynthetic capabilities, associated with the predominance of the genes encoding the proteins involved in catabolic processes. Multiple single-nucleotide substitutions causing intraspecific polymorphism of Mycoplasma hominis were found. The genes encoding the efflux systems – ABC transporters (the ATP-binding cassette superfamily) and proteins of the MATE (multidrug and toxic compound extrusion) family – were identified. The molecular mechanism of ciprofloxacin resistance of the Mycoplasma hominis M45 and M57 isolates was found to be associated with the Ser83Leu substitution in DNA gyrase subunit A. In the Mycoplasma hominis MH1866 isolate it was related to the Lys144Arg substitution in topoisomerase IV subunit A.
  • Keywords
    MATE , ABC transporters , gyrA and parC genes , antibiotic resistance mechanisms , genome structure , Mycoplasma hominis
  • Journal title
    Acta Naturae
  • Serial Year
    2020
  • Full Text URL
    www.ncbi.nlm.nih.gov/pmc/articles/PMC7245959/pdf/AN20758251-12-01-056.pdf
  • Record number

    2617143

    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=10&DC=2617143