Title of article :
Allogeneic mesenchymal stem cells and xenogenic platelet rich plasma, associated or not, in the repair of bone failures in rabbits with secondary osteoporosis
Author/Authors :
Rocha, Mariá Andrade de Carvalho Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Silva, Laís Meireles Costa Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Oliveira, Wellson Andrade de Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Bezerra, Dayseanny de Oliveira Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Silva, Gustavo Cardoso da Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Silva, Lucilene dos Santos Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Medeiros, Brenda Lurian do Nascimento Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Baêta, Sílvia de Araújo França Department of Clinical and Veterinary Surgery - Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Carvalho, Maria Acelina Martins de Department of Veterinary Morphology - Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Martins, Napoleão Department of Clinical and Veterinary Surgery - Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil
Abstract :
Purpose:
To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism.
Methods:
Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment.
Results:
A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others.
Conclusion:
The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p<0.01), when compared to the other treatments.
Keywords :
Mesenchymal Stromal Cells , Platelet-Rich Plasma , Osteoporosis , Rabbits
