Multimodality Molecular Imaging-Guided Tumor Border Delineation and Photothermal Therapy Analysis Based on Graphene Oxide-Conjugated Gold Nanoparticles Chelated with Gd

Tumor cell complete extinction is a crucial measure to evaluate antitumor efcacy. Te difculties in defning tumor margins and fnding satellite metastases are the reason for tumor recurrence. A synergistic method based on multimodality molecular imaging needs to be developed so as to achieve the complete extinction of the tumor cells. In this study, graphene oxide conjugated with gold nanostars and chelated with Gd through 1,4,7,10-tetraazacyclododecane-N,N ,N,N -tetraacetic acid (DOTA) (GO-AuNSDOTA-Gd) were prepared to target HCC-LM3-fLuc cells and used for therapy. For subcutaneous tumor, multimodality molecular imaging including photoacoustic imaging (PAI) and magnetic resonance imaging (MRI) and the related processing techniques were used to monitor the pharmacokinetics process of GO-AuNS-DOTA-Gd in order to determine the optimal time for treatment. For orthotopic tumor, MRI was used to delineate the tumor location and margin in vivo before treatment.Ten handheld photoacoustic imaging system was used to determine the tumor location during the surgery and guided the photothermal therapy.Te experiment result based on orthotopic tumor demonstrated that this synergistic method could efectively reduce tumor residual and satellite metastases by 85.71% compared with the routine photothermal method without handheld PAI guidance. Tese results indicate that this multimodality molecular imaging-guided photothermal therapy method is promising with a good prospect in clinical application.