Author/Authors :
Zhang, Xiao Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Hu, Fan Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Liu, Chunbao Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Yin, Lianglan Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Zhang, Yingying Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Zhang, Yongxue Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Lan, Xiaoli Department of Nuclear Medicine, union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China
Abstract :
Vascular cell adhesion molecule 1 (VCAM-1) is overexpressed in varieties of cancers. Tis study aimed to evaluate the application
of a single chain variable fragment (scFv) of anti-VCAM-1 antibody labeled with 99mTc as a possible imaging agent in several
tumors. VCAM-1 scFv was labeled with 99mTc using succinimidyl 6-hydrazinium nicotinate hydrochloride, and 99mTc-HYNICVCAM-1scFv was successfully synthesized with a high radiolabeling yield. VCAM-1 expression was evaluated in six cell lines
by immunofuorescence staining. In vitro binding assays showed diferent binding afnities of 99mTc-HYNIC-VCAM-1scFv in
diferent tumor cell lines, with high uptake in B16F10 melanoma and HT1080 fbrosarcoma cells, which was consistent with
immunofuorescence staining results. In vivo SPECT planar imaging demonstrated that B16F10 and HT1080 tumors could be
clearly visualized. Less intense uptake was observed in human SKOV3.ip ovarian tumor, and weak uptake was observed in human
A375m melanoma, MDA-MB-231 breast cancer, and 786-O renal tumors. Tese fndings were confrmed by biodistribution and
immunofuorescence studies. High uptake by B16F10 tumors was inhibited by excess unlabeled VCAM-1scFv.
99mTc-HYNIC-VCAM1scFv, which selectively binds to VCAM-1, can provide a qualitative and semiquantitative method for noninvasive evaluation of
VCAM-1 expression by tumors.
