Author/Authors :
Ma, Weiqiong Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Xie, Qi Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Zhang, Baolin School of Materials Science and Engineering - Guilin University of Technology - Guilin - Guangxi, China , Chen, Huixian Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Tang, Jianyi Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Lei, Zhengxian Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Wu, Minyi Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Zhang, Dingxuan Second Afliated Hospital of South China University of Technology - Guangzhou - Guangdong, China , Hu, Jiani Wayne State University - Detroit1, USA
Abstract :
Magnetic resonance imaging (MRI) combined with contrast agents is believed to be useful for stem cell tracking in vivo, and the aim
of this research was to investigate the biosafety and neural induction of SD rat-originated adipose derived stem cells (ADSCs) using
cationic superparamagnetic iron oxide (SPIO) nanoparticle which was synthesized by the improved polyol method, in order to allow
visualization using in vitro MRI. The scan protocols were performed with T2-mapping sequence; meanwhile, the ultrastructure
of labeled cells was observed by transmission electron microscopy (TEM) while the iron content was measured by inductively
coupled plasma-atomic emission spectrometry (ICP-AES). After neural induction, nestin and NSE (neural markers) were obviously
expressed. In vitro MRI showed that the cationic PEG/PEI-modifed SPIO nanoparticles could achieve great relaxation performance
and favourable longevity. and the ICP-AES quantifed the lowest iron content that could be detected by MRI as 1.56∼1.8 pg/cell.
Tis study showed that the cationic SPIO could be directly used to label ADSCs, which could then inductively diferentiate into
nerve and be imaged by in vitro MRI, which would exhibit important guiding signifcance for the further in vivo MRI towards
animal models with neurodegenerative disorders.
