Author/Authors :
Li, Hong-Bin Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Li, Na Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Wen, Shu-Ran Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Qiang, Ming-Yue Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Yang, Zheng-Hui Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Dong, Tian-Xiang Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Li, Yu-Ye Department of Dermatology and Venereology - First Affiliated Hospital of Kunming Medical University, Yunnan, China , Kuang, Yi-Qun NHC Key Laboratory of Drug Addiction Medicine - First Affiliated Hospital of Kunming Medical University - Kunming Medical University, Yunnan, China
Abstract :
Objective
Candida glabrata (C. glabrata) causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during C. glabrata infection.
Methods
RAW264.1 macrophages were treated with MCF, ICZ, or AmB and then challenged with C. glabrata. Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis.
Results
The activity of SOD was significantly increased in RAW264.1 cells that phagocytized C. glabrata and reached a peak level at 6 hours (P < 0.05). ICZ and AmB did not affect the SOD activity in cells that phagocytized C. glabrata versus that in untreated macrophage. C. glabrata stimulated macrophages to secrete cytokines. Neither ICZ nor AmB affected the secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), or tumor necrosis factor-α (TNF-α) by C. glabrata-infected macrophages. However, MCF downregulated the secretion of TNF-α by infected macrophages and reduced the SOD activity of C. glabrata compared with those in untreated controls.
Conclusion
Echinocandins may increase their antifungal efficacy by altering the innate immune response of macrophages and attenuating antioxidants of this organism.
