Author/Authors :
Lwin, Thet-Thet Allied Health Sciences - Kitasato University, Sagamihara, Japan , Yoneyama, Akio Allied Health Sciences - Kitasato University, Sagamihara, Japan , Hara, Atsuko Graduate School of Medical Sciences - Kitasato University, Sagamihara, Japan , Ohbu, Makoto Allied Health Sciences - Kitasato University, Sagamihara, Japan , Maruyama, Hiroko Allied Health Sciences - Kitasato University, Sagamihara, Japan , Taguchi, Masaya Graduate School of Medical Sciences - Kitasato University, Sagamihara, Japan , Esashi, Shogo Graduate School of Medical Sciences - Kitasato University, Sagamihara, Japan , Matsushima, Tsubasa Graduate School of Medical Sciences - Kitasato University, Sagamihara, Japan , Terazaki, Kei Graduate School of Medical Sciences - Kitasato University, Sagamihara, Japan , Hyodo, Kazuyuki High Energy Accelerator Research Organization, Tsukuba, Japan , Takeda, Tohoru Allied Health Sciences - Kitasato University, Sagamihara, Japan
Abstract :
Background
Crystal X-ray interferometer-based phase-contrast X-ray computed tomography (C-PCCT) enables the depiction of internal structures of biological tissue without contrast agents.
Purpose
To determine the advantage of this technique in visualizing detailed morphological structures of a rare spontaneous brain tumor in an aged rat.
Material and Methods
An aged rat’s spontaneous brain tumor was imaged by C-PCCT without contrast agent. Three-dimensional (3D) images of the tumor microvasculature were reconstructed and compared with pathological pictures.
Results
C-PCCT depicted the tumor’s various pathological features clearly, e.g. its cell density and vasculature, and blood clots caused by hemorrhaging and/or hematomas. The obtained images resembled pathological pictures with a magnification of ×20 and were used to reconstruct 3D images of the tumor vascularity up to approximately 26 µm in diameter.
Conclusion
Since C-PCCT is able to depict various pathological conditions, it might be useful for cancer research.
Keywords :
Crystal X-ray interferometer-based phase-contrast X-ray CT (C-PCCT) , computed tomography (CT) , aged rat , spontaneous brain tumor
