Author/Authors :
Campelo, Mariana Barbosa Dias Universidade do Vale do Paraíba (UNIVAP), Sao Jose dos Campos-SP, Brazil , Santos, Joelita de Alencar Fonseca UNIVAP, Sao Jose dos Campos-SP, Brazil , Maia, Antonio Luiz Martins Universidade Estadual do Piauí (UESPI), TeresinaPI, Brazil , Ferreira, Daniel Cabral Leão UESPI, Teresina-PI, Brazil , Sant’Anna, Luciana Barros UNIVAP, Sao Jose dos Campos-SP, Brazil , Oliveira, Rauirys Alencar de Department of Physiotherapy - UESPI, Teresina-PI, Brazil , Maia, Leonardo Fonseca Department of Medicine - UFPI, Teresina-PI, Brazil , Arisawa, Emilia Ângela Loschiavo UNIVAP, Sao Jose dos Campos-SP, Brazil
Abstract :
Purpose:
To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.
Methods:
Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.
Results:
The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).
Conclusion:
Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.
Keywords :
Wound Healing , Amnion , Collagen , Inflammation , Rats
