Author/Authors :
Silva, Samanta Sarmento da School of Medicine - Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil , Peterson, Guilherme Eckert School of Medicine - Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil , Amantéa, Sérgio Luis Pediatric Department - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil , Miorelli, Patrícia School of Medicine - UFRGS, Porto Alegre-RS, Brazil , Ulbrich, Jane Maria Department of Pathology - UFRGS, Porto Alegre-RS, Brazil , Roesch, Eliane Hospital de Clínicas de Porto Alegre (HCPA), Brazil , Sanches, Paulo Roberto HCPA, Porto Alegre-RS, Brazil , Fraga, Jose Carlos Department of Surgery - UFRGS, Porto Alegre-RS, Brazil
Abstract :
Purpose:
To evaluate the concentration of transforming growth factor beta 1 (TGFB1) levels in a rat pleural effusion obtained by inoculation of intrapleural bacteria or turpentine through thoracentesis.
Methods:
Thirty-Nine Wistar rats were divided into three groups: Staphylococcus aureus (SA, n = 17); Streptococcus pneumoniae (SP, n = 12); and turpentine (control, n = 10). Pleural fluid was collected through ultrasound-guided thoracentesis 12 h, 24 h, and 36 h after instillation of bacteria or turpentine. Levels of TGFB1 were measured in pleural fluid.
Results:
At 12 h, mean TGFB1concentrations were 5.3450 pg/mL in the SA group, 5.3449 pg/mL in the SP group, and 5.3450 pg/mL in controls. At 24 h, they were 4.6700 pg/mL in the SA group, 4.6700 pg/mL in the SP group, and 4.6700 pg/mL in controls. At 36 h, they were 4.6699 pg/mL in the SA group and in control. No difference was observed among the groups in mean TGFB1concentration (p = 0.12); however, a significant intragroup reduction in mean TGFB1 was observed between 12 and 24 h (p < 0.01).
Conclusion:
The transforming growth factor beta 1 concentrations were not useful as a diagnostic tool or an early marker of infected pleural effusion.
Keywords :
Empyema , Pleural , Cytokines , Transforming Growth Factor beta1 , Rats
