Title of article
The role of oxymatrine in regulating TGF-β1 in rats with hepatic fibrosis
Author/Authors
Wu, Jing Ningxia Medical University, Yinchuan, China , Pan, Lin Ningxia Medical University, Yinchuan, China , Jin, Xueqin Ningxia Medical University, Yinchuan, China , Li, Weihua Ningxia Medical University, Yinchuan, China , Li, Hongbing Ningxia Medical University, Yinchuan, China , Chen, Jianmao Ningxia Medical University, Yinchuan, China , Yang, Wen Ningxia Medical University, Yinchuan, China
Pages
9
From page
207
To page
215
Abstract
Purpose:
To investigate whether oxymatrine (OMT) prevents hepatic fibrosis in rats by regulating liver transforming growth factor β1 (TGF-β1) level.
Methods:
Hepatic fibrosis was induced in rats by thioacetamide (TAA). Blood was collected at the end of week 12 to determine the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutathione (GSH). Changes in liver tissue were observed after hematoxylin-eosin (HE) staining.
Results:
Fibrosis was confirmed by Masson’s collagen staining. Liver TGF-β1 level was determined by ELISA. OMT significantly reduced serum ALT and AST but increased GSH levels in rats with hepatic fibrosis. Moreover, it significantly improved liver histology in rats with TAA-induced hepatic fibrosis. It significantly decreased liver TGF-β1 level compared to that in the untreated group. It also significantly reduced collagen deposition in rats.
Conclusion:
Oxymatrine is effective in protecting rats from thioacetamide-induced hepatic fibrosis by regulating TGF-β1 expression.
Keywords
Thioacetamide , Fibrosis , Liver , Transforming Growth Factor beta 1 , Rats
Journal title
Acta Cirurgica Brasileira
Serial Year
2018
Record number
2619878
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