Author/Authors :
Yavuz, Erkan Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Ercan, Gulcin Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Karagulle, Onur Olgac Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Bayrak, Busra Yaprak Department of Pathology - University of Health Science Derince Training and Research Hospital, Kocaeli, Turkey , Biricik, Aytac Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Ercetin, Candas Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Gokcek, Berk Department of General Surgery - University of Health Science Okmeydanı Training and Research Hospital, Istanbul, Turkey , Yigitbas, Hakan Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Kusaslan, Ramazan Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Celik, Atilla Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey , Gulcicek, Osman Bilgin Department of General Surgery - University of Health Science Bagcilar Training and Research Hospital, Istanbul, Turkey
Abstract :
Purpose:
To investigate the prophylactic and therapeutical effects of sildenafil in a model of acute radiation proctitis (ARP).
Methods:
All experimental procedures of this study was examined by histopathological, immunohistochemical and transmission electron microscopic analysis.
Results:
Our histopathological evaluations indicated significant increases in lesion severity, cryptic apsis, cryptitis, cryptic distortion, reactive atypia and infiltration depth of the control (proctitis) group. While the prophylaxis group and the treatment group had significantly lower scores. High-dose group showed similar results as prophylaxis group. Histopathological findings of the prophylaxis group was more significant than the treatment group. Immunoreactivities of IL-1β, FGF-2, TNF- α and HIF-1α increased in the control group especially in the epithelial and cryptic regions. On the contrary, sildenafil application caused significant decreases of inflammatory markers in all treatment groups, specifically better results in the prophylaxis group.
Conclusion:
The sildenafil has anti-inflammatory effects on ARP, as well as protective effects against ARP and the protective effect of sildenafil surpasses its therapeutic effect histopathologically.
Keywords :
Sildenafil Citrate , Proctitis , Inflammation , Ultrastructure , Rats
