Author/Authors :
Onk, Didem Department of Anesthesiology and Reanimation - Faculty of Medicine - Erzincan University, Turkey , Onk, Oruç Alper Department of Cardiovascular Surgery - Faculty of Medicine - Erzincan University, Turkey , Erol, Hüseyin Serkan Department of Biochemistry - Faculty of Veterinary - Atatürk University, Erzurum, Turkey , Özkaraca, Mustafa Department of Pathology - Faculty of Veterinary - Atatürk University, Erzurum, Turkey , Çomaklı, Selim Department of Pathology - Faculty of Veterinary - Atatürk University, Erzurum, Turkey , Ayazoğlu, Tülin Akarsu Department of Anesthesiology and Reanimation - Göztepe Training and Research Hospital, Istanbul, Turkey , Kuyrukluyıldız, Ufuk Department of Anesthesiology and Reanimation - Faculty of Medicine - Erzincan University, Erzincan, Turkey , Ünver, Süheyla Department of Anesthesiology and Reanimation - Faculty of Medicine - Erzincan University, Turkey
Abstract :
Purpose:
To investigate the effects of melatonin on antioxidant capacity, inflammation and apoptotic cell death (through expression of cleaved-caspase 3) in lung tissue samples of diabetic rats.
Methods:
Thirty male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control group) was made up of healthy rats. Group 2 (diabetes group) received streptozotocin at a dose of 50 mg/kg/day for 5 days.Group 3 (diabetes plus melatonin group) received streptozotocin at a dose of 50 mg/kg/day for 5 days and then they received melatonin at a dose of 20 mg/kg/day between 28thand 35thdays of the study.
Results:
Tissue MDA and MPO levels were found to be significantly higher in diabetes group compared to control group (p<0.05) whilst administration of melatonin was found to significantly lower this increase down to normal levels (p<0.05). Bronchus associated lymphoid tissue (BALT) was more severe in diabetics whereas administration of melatonin alleviated this hyperplasia. Cleaved caspase 3 activity was severe in hyperplastic BALT in diabetic rats however in lowered down to moderate level when melatonin was administered.
Conclusion:
The melatonin caused an increase in antioxidant capacity and decreased the expression of cleaved-caspase 3.
