Author/Authors :
Brito, Felipe Nunes School of Medicine - Universidade Federal University do Pará (UFPA), Belem-PA, Brazil , Vendramin, Fabiel Spani UFPA, Belem-PA, Brazil , Lopes, Cinthia Távora de Albuquerque UFPA, Belem-PA, Brazil , Costa, Mayra Pauline Ribeiro UFPA, Belem-PA, Brazil , Ohashi, Otávio Mitio UFPA, Belem-PA, Brazil , Maia, José Guilherme Soares Universidade Federal do Oeste do Pará (UFOPA), Santarem-PA, Brazil , Ferreira, Lydia Masako Universidade Federal de São Paulo (UNIFESP), Brazil , Silva, Joyce Kelly do Rosário da UFPA, Belem-PA, Brazil , Miranda, Moysés dos Santos UFPA, Belem-PA, Brazil
Abstract :
Purpose:
To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells.
Methods:
Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3.
Results:
Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells.
Conclusion:
Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.
Keywords :
Stem Cells , Adipose Tissue , Cell Proliferation , Thymol
