Title of article :
Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
Author/Authors :
Mensch, Barbara S. Population Council - New York - New York, United States , Kalibala, Sam Population Council - Washington - District of Columbia, United States , Hewett, Paul C. Population Council - Washington - District of Columbia, United States , Nsobya, Sam L. Department of Pathology - School of Biomedical Science - Makerere University - Kampala, Uganda
Abstract :
This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.
Keywords :
Kalon , dried blood spots , DBS , Herpes Simplex Virus Type 2 , HSV-2
Journal title :
African Journal of Laboratory Medicine
