Author/Authors :
Mlisana, Koleka National Health Laboratory Services - Inkosi Albert Luthuli Central Hospital - Durban - KwaZulu Natal, South Africa , Mahomed, Sharana National Health Laboratory Services - Inkosi Albert Luthuli Central Hospital - Durban - KwaZulu Natal, South Africa , Dlamini-Mvelase, Nomonde R. National Health Laboratory Services - Inkosi Albert Luthuli Central Hospital - Durban - KwaZulu Natal, South Africa , Dlamini, Moses National Health Laboratory Services - Inkosi Albert Luthuli Central Hospital - Durban - KwaZulu Natal, South Africa
Abstract :
For the optimal recovery of Mycobacterium tuberculosis from the BACTEC™ Mycobacterium Growth Indicator Tube 960™ system, an incubation period of 42–56 days is recommended by the manufacturer. Due to logistical reasons, it is common practice to follow an incubation period of 42 days. We undertook a retrospective study to document positive Mycobacterium Growth Indicator Tube cultures beyond the 42-day incubation period. In total, 98/110 (89%) were positive for M. tuberculosis complex. This alerted us to M. tuberculosis growth detection failure at 42 days.
Keywords :
Failure , BACTEC ™ MGIT 960 ™ , detect Mycobacterium tuberculosis complex , 42-day incubation period
