Title of article :
Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
Author/Authors :
Potgieter, Joachim J.C. Department of Haematology - Faculty of Health Sciences - University of Pretoria - Pretoria, South Africa , Senamela, Tshiphiri Department of Haematology - Faculty of Health Sciences - University of Pretoria - Pretoria, South Africa , Kock, Marleen Department of Haematology - Faculty of Health Sciences - University of Pretoria - Pretoria, South Africa , Becker, Piet Department of Haematology - Faculty of Health Sciences - University of Pretoria - Pretoria, South Africa
Abstract :
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.
Keywords :
Detection , Janus kinase 2 V617F mutation , locked nucleic-acid , real-time polymerase chain reaction assay , PCR , JAK2 V617F , Myeloproliferative neoplasms (MPNs)
Journal title :
African Journal of Laboratory Medicine
