Author/Authors :
Ismail, Nazir A. Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Omar, Shaheed V. Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Joseph, Lavania Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Said, Halima M. Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Ismail, Farzana Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Ismail, Nabila Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa , Gwala, Thabisile L. Centre for Tuberculosis - World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases - National Health Laboratory Service - Johannesburg, South Africa
Abstract :
South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.
