Author/Authors :
Yuan,Chunyu Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Bu, Wenbo Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Li, Li Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Zhang, Mengli Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Chen, Kun Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Fang, Fang Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Li, Min Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Chen, Xu Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China , Gu, Heng Institute of Dermatology - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs - Chinese Academy of Medical Science & Peking union Medical College, Nanjing, China
Abstract :
Introduction
Long non-coding RNA (lncRNA) plays a key role in various disorders. However, its role in keloid is still unclear.
Aim
We explored differentially expressed (DE) lncRNAs and mRNAs between keloid tissue (KT)s and normal tissue (NT)s, as well as keloid fibroblast (KFB)s and normal fibroblast (NFB)s, respectively.
Material and methods
We use KTs and NTs from the chest of 5 patients, and 3 pairs of KFBs and NFBs, to perform microarray respectively. Gene ontology and pathway analyses were conducted by online software DAVID (Database for Annotation, Visualization and Integrated Discovery). The validation of targeted lncRNAs were conducted by qRT-PCR in enlarged samples (79 KTs and 21 NTs).
Results
We identified 3680 DE-lncRNAs in tissue essay, and 1231 DE-lncRNAs in cell essay. Furthermore, we found that many lncRNAs and their relative mRNAs were regulated simultaneously in keloid. We identified that ENST00000439703 and uc003jox.1 were up-regulated in both of the above essays through comparing the results of lncRNA screening between tissue essay and cell essay; the results were confirmed through qRT-PCR in enlarged samples.
Conclusions
Our study demonstrates that numerous lncRNAs are involved in the pathogenesis and development of the keloid.
Keywords :
long non-coding RNA , keloid , fibroblast , mRNA , pathway
