Author/Authors :
Nascimento, Lízia Daniela e Silva Universidade do Vale do Paraíba (UNIVAP), Sao Jose dos Campos-SP, Brazil , Nicolau, Renata Amadei Universidade do Vale do Paraíba (UNIVAP), Sao Jose dos Campos-SP, Brazil , Maia, Antônio Luiz Martins Health Sciences Center - UESPI, Teresina-PI, Brazil , Santos, José Zilton Lima Verde Center of Health Sciences, Medicine and Physical Therapy - UESPI, Teresina-PI. Brazil , Fonseca, Khetyma Moreira Universidade Federal do Ceará (UFC), Fortaleza-CE, Brazil , Ferreira, Danniel Cabral Leão UESPI, Teresina-PI, Brazil , Sousa, Rayssilane Cardoso de Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil , Viana, Vicente Galber Freitas IFPI, Teresina-PI, Brazil , Carvalho, Luiz Fernando Meneses IFPI, Teresina-PI, Brazil , Figueredo-Silva, José Health Sciences Center - UESPI, Teresina-PI, Brazil
Abstract :
Purpose:
To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair.
Methods:
Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically.
Results:
Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107).
Conclusion:
Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.
