Title of article :
Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells
Author/Authors :
Hesari ، Roya Institute of Materials and Biomaterials , Keshvarinia ، Mina Department of Biology - Islamic Azad University, Science and Research Branch , Kabiri ، Mahboubeh Department of Biotechnology - College of Science - University of Tehran , Rad ، Iman Stem Cell Technology Research Center , Parivar ، Kazem Department of Biology - Islamic Azad University, Science and Research Branch , Hoseinpoor ، Hoorieh Stem Cell Technology Research Center , Tavakoli ، Rezvan Stem Cell Technology Research Center , Soleimani ، Masoud Hematology Department - Faculty of Medical Sciences - Tarbiat Modares University , Kouhkan ، Fatemeh Stem Cell Technology Research Center , Zamanluee ، Soheila Tissue Engineering and Regenerative Medicine Institute - Islamic Azad University, Tehran Central Branch , Hanaee-Ahvaz ، Hana Stem Cell Technology Research Center
Abstract :
Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays. Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.
Keywords :
Calcium deposition , Chondrogenesis , Mesenchymal stem cells , Transforming growth factor , beta , Platelet rich plasma
Journal title :
Bioimpacts
Journal title :
Bioimpacts
